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Image Search Results
Journal: BMC Plant Biology
Article Title: A DUF-246 family glycosyltransferase-like gene affects male fertility and the biosynthesis of pectic arabinogalactans
doi: 10.1186/s12870-016-0780-x
Figure Lengend Snippet: Characterization of RG-I in PAGR- overexpressing Arabidopsis lines and NbPAGR -silenced N. benthamiana . Size exclusion chromatography using a Superdex 200 column of RG-I from NbPAGR -silenced and control N. benthamiana cell walls ( a ) and RG-I from 35S::PAGR-YFP lines and the wild type ( b ). RG-I from NbPAGR- silenced lines eluted later than the control indicating a decrease in the average molecular weight of RG-I domains. RG-I from PAGR-overexpressing Arabidopsis lines eluted slightly earlier than RG-I from the wild type. c ELISA analysis of size exclusion chromatography-purified RG-I with plant-glycan directed monoclonal antibodies
Article Snippet: Purified RG-I samples were dissolved in water and were coated onto
Techniques: Size-exclusion Chromatography, Control, Molecular Weight, Enzyme-linked Immunosorbent Assay, Purification, Glycoproteomics, Bioprocessing